Experiment set4IT050 for Paraburkholderia graminis OAS925

R2A outgrowth control

Group: r2a outgrowth control
Media: R2A
Culturing: Burkholderia_OAS925_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Marta on 20-May-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 3 genes in this experiment

SEED Subsystems

Subsystem	#Specific
De Novo Pyrimidine Synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyrimidine metabolism
• Alanine and aspartate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UMP biosynthesis II	6	6	1
UMP biosynthesis I	6	6	1
UMP biosynthesis III	6	5	1
superpathway of pyrimidine ribonucleotides de novo biosynthesis	9	9	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	18	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	44	1