Experiment set4IT048 for Dinoroseobacter shibae DFL-12
Adenosine nitrogen source
Group: nitrogen sourceMedia: DinoMM_noNitrogen_HighNutrient + Adenosine (1 mM), pH=7.2
Culturing: Dino_ML3, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 2.7 generations
By: Adam on 5/14/2014
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 1.37 g/L Sodium acetate, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 2 genes in this experiment
For nitrogen source Adenosine in Dinoroseobacter shibae DFL-12
For nitrogen source Adenosine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Deoxyribose and Deoxynucleoside Catabolism | 1 |
| Purine conversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| purine ribonucleosides degradation | 6 | 6 | 2 |
| purine deoxyribonucleosides degradation II | 3 | 3 | 1 |
| adenine and adenosine salvage V | 3 | 2 | 1 |
| 2-deoxy-α-D-ribose 1-phosphate degradation | 3 | 2 | 1 |
| superpathway of purine deoxyribonucleosides degradation | 7 | 6 | 2 |
| adenine and adenosine salvage III | 4 | 4 | 1 |
| purine deoxyribonucleosides degradation I | 4 | 4 | 1 |
| adenosine nucleotides degradation II | 5 | 5 | 1 |
| superpathway of pyrimidine deoxyribonucleosides degradation | 6 | 5 | 1 |
| purine nucleotides degradation II (aerobic) | 11 | 11 | 1 |