Experiment set4IT045 for Pseudomonas fluorescens SBW25-INTG

Growth at pH6 and carbon source D-Glucose

Group: pH
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM) + MES (40 mM), pH=6
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 2 genes in this experiment

For pH D-Glucose in Pseudomonas fluorescens SBW25-INTG

For pH D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
2-Ketogluconate Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Glutathione metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glucose degradation (oxidative)	5	5	1