Experiment set4IT045 for Desulfovibrio vulgaris Miyazaki F

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MoYLS4 with 2-Furfuraldehyde 0.0625 %

Group: stress
Media: MoYLS4 + 2-Furfuraldehyde (0.0625 vol%), pH=7.2
Culturing: Miya_ML3_a, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Jen on 1/20/2015
Media components: 1 g/L Yeast Extract, 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate, Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 2 genes in this experiment

For stress 2-Furfuraldehyde in Desulfovibrio vulgaris Miyazaki F

For stress 2-Furfuraldehyde across organisms

SEED Subsystems

Subsystem #Specific
Alanine biosynthesis 1
Nitrogen fixation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine biosynthesis III 1 1 1
L-cysteine degradation IV 1 1 1
cytidylyl molybdenum cofactor sulfurylation 2 1 1
bis(guanylyl molybdopterin) cofactor sulfurylation 3 1 1
thiazole component of thiamine diphosphate biosynthesis II 7 5 2
superpathway of L-alanine biosynthesis 4 3 1
tRNA-uridine 2-thiolation (yeast mitochondria) 4 1 1
tRNA-uridine 2-thiolation (mammalian mitochondria) 4 1 1
tRNA-uridine 2-thiolation (thermophilic bacteria) 5 2 1
[2Fe-2S] iron-sulfur cluster biosynthesis 10 2 2
superpathway of thiamine diphosphate biosynthesis II 11 9 2
thiazole component of thiamine diphosphate biosynthesis I 6 4 1
molybdopterin biosynthesis 6 2 1
tRNA-uridine 2-thiolation (cytoplasmic) 8 1 1
superpathway of thiamine diphosphate biosynthesis I 10 8 1
tRNA-uridine 2-thiolation and selenation (bacteria) 11 2 1