Experiment set4IT044 for Rhodanobacter denitrificans FW104-10B01

R2A with sodium bromide 250 mM

Group: stress
Media: R2A + sodium bromide (250 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans Carlson and Trenton Owens on 22-Jun-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 3B H3

Specific Phenotypes

For 10 genes in this experiment

For stress sodium bromide in Rhodanobacter denitrificans FW104-10B01

For stress sodium bromide across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial Cytoskeleton	1
Glutathione: Non-redox reactions	1
Methylglyoxal Metabolism	1
Peptidoglycan Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• Pyruvate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
methylglyoxal degradation VIII	3	2	1
methylglyoxal degradation I	3	2	1
superpathway of methylglyoxal degradation	8	4	1