Experiment set4IT044 for Rhodanobacter denitrificans FW104-10B01
R2A with sodium bromide 250 mM
Group: stressMedia: R2A + sodium bromide (250 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans Carlson and Trenton Owens on 06/22/2021
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 3B H3
Specific Phenotypes
For 10 genes in this experiment
For stress sodium bromide in Rhodanobacter denitrificans FW104-10B01
For stress sodium bromide across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Bacterial Cytoskeleton | 1 |
| Glutathione: Non-redox reactions | 1 |
| Methylglyoxal Metabolism | 1 |
| Peptidoglycan Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Ascorbate and aldarate metabolism
- Ubiquinone and menaquinone biosynthesis
- Nucleotide sugars metabolism
- Glycosaminoglycan degradation
- Lipopolysaccharide biosynthesis
- Pyruvate metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| methylglyoxal degradation I | 3 | 2 | 1 |
| methylglyoxal degradation VIII | 3 | 2 | 1 |
| superpathway of methylglyoxal degradation | 8 | 4 | 1 |