Experiment set4IT042 for Pseudomonas fluorescens SBW25-INTG
Growth at pH9 and carbon source D-Glucose
Group: pHMedia: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM) + AMPSO (40 mM), pH=9
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20
Specific Phenotypes
For 14 genes in this experiment
For pH D-Glucose in Pseudomonas fluorescens SBW25-INTG
For pH D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Fructose and Mannose Inducible PTS | 1 |
| Fructose utilization | 1 |
| Lysine degradation | 1 |
| Mannitol Utilization | 1 |
| Pentose phosphate pathway | 1 |
| Potassium homeostasis | 1 |
| tRNA processing | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Ascorbate and aldarate metabolism
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Biosynthesis of plant hormones
- Glycolysis / Gluconeogenesis
- Pentose phosphate pathway
- Pentose and glucuronate interconversions
- Fructose and mannose metabolism
- Galactose metabolism
- Pyrimidine metabolism
- Lysine degradation
- Tryptophan metabolism
- Glutathione metabolism
- Aminosugars metabolism
- Biosynthesis of alkaloids derived from histidine and purine
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| UDP-α-D-glucuronate biosynthesis (from UDP-glucose) | 1 | 1 | 1 |
| indole-3-acetate biosynthesis III (bacteria) | 2 | 2 | 1 |
| pseudouridine degradation | 2 | 1 | 1 |
| UDP-α-D-xylose biosynthesis | 2 | 1 | 1 |
| pentose phosphate pathway (oxidative branch) I | 3 | 3 | 1 |
| L-lysine degradation IV | 5 | 5 | 1 |
| superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis | 6 | 4 | 1 |
| pentose phosphate pathway | 8 | 8 | 1 |
| UDP-sugars interconversion | 9 | 2 | 1 |
| teichuronic acid biosynthesis (B. subtilis 168) | 9 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 11 | 1 |
| superpathway of glucose and xylose degradation | 17 | 16 | 1 |
| superpathway of L-lysine degradation | 43 | 17 | 1 |