Experiment set4IT040 for Rhodospirillum rubrum S1H

L-Malic Acid carbon source and N2; anaerobic; high light

Group: anaerboic nitrogen source
Media: MELiSSA_defined_noCarbon_noNitrogen_phosphatebuffered + N2 (90 % atm) + L-Malic acid (20 mM), pH=7
Culturing: Rubrum_ML4a, serum bottle, Anaerobic, at 30 (C)
By: Kelly Wetmore; Jordan Baker on 4/25/19
Media components: 0.051 mM Manganese (II) chloride tetrahydrate, 0.0811 mM Magnesium Sulfate Heptahydrate, 3.801 mM Sodium sulfate, 0.34 mM Calcium chloride dihydrate, 0.068 mM EDTA, 3.601 mM Potassium phosphate monobasic, 0.072 mM Iron (II) sulfate heptahydrate, 50 mM Sodium bicarbonate, 2.985 mM Potassium phosphate dibasic, 1.902 uM Nickel (II) sulfate hexahydrate, 0.348 uM Zinc sulfate heptahydrate, 0.02 uM Copper (II) sulfate pentahydrate, 1.623 uM Boric Acid, 0.207 uM Sodium Molybdate Dihydrate, 0.06 uM biotin, 10 mM Potassium phosphate monobasic, 0.597 mM Potassium phosphate dibasic

Specific Phenotypes

For 3 genes in this experiment

For anaerboic nitrogen source N2 in Rhodospirillum rubrum S1H

For anaerboic nitrogen source N2 across organisms

SEED Subsystems

Subsystem	#Specific
Nitrogen fixation	3

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tetrachloroethene degradation
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
nitrogen fixation I (ferredoxin)	1	1	1