Experiment set4IT039 for Rhodanobacter denitrificans FW104-10B01
R2A with Potassium Chloride 250 mM
Group: stressMedia: R2A + Potassium Chloride (250 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans Carlson and Trenton Owens on 22-Jun-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 2B C3
Specific Phenotypes
For 7 genes in this experiment
For stress Potassium Chloride in Rhodanobacter denitrificans FW104-10B01
For stress Potassium Chloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Sodium Hydrogen Antiporter | 3 |
| Bacterial Cytoskeleton | 1 |
| Glutathione: Non-redox reactions | 1 |
| Methylglyoxal Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| methylglyoxal degradation I | 3 | 2 | 1 |
| methylglyoxal degradation VIII | 3 | 2 | 1 |
| superpathway of methylglyoxal degradation | 8 | 4 | 1 |