Experiment set4IT039 for Pseudomonas fluorescens FW300-N2C3

LB with methylglyoxal 0.016 vol%

Group: stress
Media: LB + methylglyoxal (0.016 vol%)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/8/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 988 B3,B4

Specific Phenotypes

For 22 genes in this experiment

For stress methylglyoxal in Pseudomonas fluorescens FW300-N2C3

For stress methylglyoxal across organisms

SEED Subsystems

Subsystem	#Specific
DNA-replication	3
DNA repair, UvrABC system	3
Glutathione-dependent pathway of formaldehyde detoxification	3
DNA repair, bacterial	2
DNA repair, bacterial RecFOR pathway	2
Arginine and Ornithine Degradation	1
DNA Repair Base Excision	1
DNA repair, bacterial DinG and relatives	1
Glutathione: Non-redox reactions	1
Lipid A modifications	1
Methionine Salvage	1
Methylglyoxal Metabolism	1
Molybdenum cofactor biosynthesis	1
Riboflavin, FMN and FAD metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Methane metabolism
• Riboflavin metabolism
• Glycolysis / Gluconeogenesis
• Fatty acid metabolism
• Methionine metabolism
• Tyrosine metabolism
• Benzoxazinone biosynthesis
• Pyruvate metabolism
• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Retinol metabolism
• Diterpenoid biosynthesis
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
acetaldehyde biosynthesis I	1	1	1
formaldehyde oxidation II (glutathione-dependent)	3	3	2
ethanol degradation I	2	2	1
pyruvate fermentation to ethanol II	2	1	1
ethanol degradation II	3	3	1
L-isoleucine degradation II	3	2	1
L-leucine degradation III	3	2	1
methylglyoxal degradation I	3	2	1
pyruvate fermentation to ethanol I	3	2	1
L-valine degradation II	3	2	1
methylglyoxal degradation VIII	3	2	1
pyruvate fermentation to ethanol III	3	2	1
L-methionine degradation III	3	1	1
5'-deoxyadenosine degradation I	3	1	1
phytol degradation	4	3	1
L-tyrosine degradation III	4	2	1
salidroside biosynthesis	4	2	1
L-phenylalanine degradation III	4	2	1
5'-deoxyadenosine degradation II	4	1	1
flavin biosynthesis I (bacteria and plants)	9	8	2
flavin biosynthesis III (fungi)	9	7	2
ethanolamine utilization	5	5	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
acetylene degradation (anaerobic)	5	4	1
protein S-nitrosylation and denitrosylation	5	3	1
6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia)	5	3	1
(S)-propane-1,2-diol degradation	5	2	1
phenylethanol biosynthesis	5	2	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation II	5	2	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation III	5	2	1
superpathway of C1 compounds oxidation to CO2	12	5	2
noradrenaline and adrenaline degradation	13	8	2
3-methylbutanol biosynthesis (engineered)	7	6	1
serotonin degradation	7	4	1
toxoflavin biosynthesis	7	3	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I	7	2	1
superpathway of methylglyoxal degradation	8	4	1
butanol and isobutanol biosynthesis (engineered)	8	3	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	5	1
flavin biosynthesis II (archaea)	10	5	1
L-methionine salvage cycle III	11	5	1
L-methionine salvage cycle II (plants)	11	3	1
L-methionine salvage cycle I (bacteria and plants)	12	4	1
superpathway of Clostridium acetobutylicum solventogenic fermentation	13	5	1
L-tryptophan degradation V (side chain pathway)	13	1	1
mixed acid fermentation	16	12	1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation	17	7	1
heterolactic fermentation	18	16	1
superpathway of anaerobic sucrose degradation	19	15	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	15	1
superpathway of N-acetylneuraminate degradation	22	15	1