Experiment set4IT039 for Bacteroides thetaiotaomicron VPI-5482

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Heparin sodium salt from porcine intestinal mucosa carbon source

Group: carbon source
Media: Varel_Bryant_medium + Heparin sodium salt from porcine intestinal mucosa (5 mg/ml)
Culturing: Btheta_ML6, tube, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 11-Oct-17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 17 genes in this experiment

For carbon source Heparin sodium salt from porcine intestinal mucosa in Bacteroides thetaiotaomicron VPI-5482

For carbon source Heparin sodium salt from porcine intestinal mucosa across organisms

SEED Subsystems

Subsystem #Specific
D-Galacturonate and D-Glucuronate Utilization 1
DNA structural proteins, bacterial 1
Iron acquisition in Vibrio 1
Ton and Tol transport systems 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
4-deoxy-L-threo-hex-4-enopyranuronate degradation 5 5 1
ergotamine biosynthesis 12 4 1
superpathway of ergotamine biosynthesis 18 5 1
tRNA charging 21 21 1
superpathway of microbial D-galacturonate and D-glucuronate degradation 31 15 1