Experiment set4IT031 for Pseudomonas fluorescens FW300-N2C3

LB with Nalidixic acid sodium salt 0.004 mg/ml

Group: stress
Media: LB + Nalidixic acid sodium salt (0.004 mg/ml)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/6/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 982 D7,D8

Specific Phenotypes

For 14 genes in this experiment

For stress Nalidixic acid sodium salt in Pseudomonas fluorescens FW300-N2C3

For stress Nalidixic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	3
DNA-replication	1
DNA Repair Base Excision	1
DNA repair, bacterial DinG and relatives	1
Glutathione: Non-redox reactions	1
Glutathione: Redox cycle	1
Lipid A modifications	1
Methylglyoxal Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Benzoxazinone biosynthesis
• Glutathione metabolism
• Pyruvate metabolism
• Diterpenoid biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glutathione-peroxide redox reactions	3	2	1
methylglyoxal degradation VIII	3	2	1
methylglyoxal degradation I	3	2	1
superpathway of methylglyoxal degradation	8	4	1