Experiment set4IT029 for Pseudomonas fluorescens FW300-N2C3
LB with Spectinomycin dihydrochloride pentahydrate 0.025 mg/ml
Group: stressMedia: LB + Spectinomycin dihydrochloride pentahydrate (0.025 mg/ml)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/6/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 982 C5,C6
Specific Phenotypes
For 8 genes in this experiment
For stress Spectinomycin dihydrochloride pentahydrate in Pseudomonas fluorescens FW300-N2C3
For stress Spectinomycin dihydrochloride pentahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Alginate metabolism | 1 |
| Mannose Metabolism | 1 |
| Multidrug Resistance Efflux Pumps | 1 |
| Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Lipopolysaccharide biosynthesis
- Fructose and mannose metabolism
- Nucleotide sugars metabolism
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- Sphingolipid metabolism
- Puromycin biosynthesis
- Lysine biosynthesis
- Glutathione metabolism
- N-Glycan biosynthesis
- O-Glycan biosynthesis
- High-mannose type N-glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Keratan sulfate biosynthesis
- Peptidoglycan biosynthesis
- Glycerolipid metabolism
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - ganglio series
- 1,4-Dichlorobenzene degradation
- Nicotinate and nicotinamide metabolism
- Carotenoid biosynthesis - General
- Zeatin biosynthesis
- Caprolactam degradation
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Flavone and flavonol biosynthesis
- Drug metabolism - other enzymes
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| poly-β-1,6-N-acetyl-D-glucosamine biosynthesis | 4 | 3 | 3 |
| D-mannose degradation I | 2 | 1 | 1 |
| D-mannose degradation II | 2 | 1 | 1 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 2 | 1 |
| mannitol biosynthesis | 3 | 1 | 1 |
| GDP-mannose biosynthesis | 4 | 4 | 1 |
| mannitol degradation II | 4 | 2 | 1 |
| 1,5-anhydrofructose degradation | 5 | 2 | 1 |
| β-(1,4)-mannan degradation | 7 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 9 | 1 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 7 | 1 |