Experiment set4IT027 for Rhodospirillum rubrum S1H

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L-Malic Acid carbon source with ammonium chloride; no light

Group: anaerboic nitrogen source
Media: MELiSSA_defined_noCarbon_noNitrogen_phosphatebuffered + Ammonium chloride (35.33 mM) + L-Malic acid (20 mM), pH=7
Culturing: Rubrum_ML4a, serum bottle, Anaerobic, at 30 (C)
By: Kelly Wetmore; Jordan Baker on 4/22/19
Media components: 0.051 mM Manganese (II) chloride tetrahydrate, 0.0811 mM Magnesium Sulfate Heptahydrate, 3.801 mM Sodium sulfate, 0.34 mM Calcium chloride dihydrate, 0.068 mM EDTA, 3.601 mM Potassium phosphate monobasic, 0.072 mM Iron (II) sulfate heptahydrate, 50 mM Sodium bicarbonate, 2.985 mM Potassium phosphate dibasic, 1.902 uM Nickel (II) sulfate hexahydrate, 0.348 uM Zinc sulfate heptahydrate, 0.02 uM Copper (II) sulfate pentahydrate, 1.623 uM Boric Acid, 0.207 uM Sodium Molybdate Dihydrate, 0.06 uM biotin, 10 mM Potassium phosphate monobasic, 0.597 mM Potassium phosphate dibasic

Specific Phenotypes

For 4 genes in this experiment

For anaerboic nitrogen source Ammonium chloride in Rhodospirillum rubrum S1H

For anaerboic nitrogen source Ammonium chloride across organisms

SEED Subsystems

Subsystem #Specific
Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway 1
Lactose and Galactose Uptake and Utilization 1
N-linked Glycosylation in Bacteria 1
Phosphate metabolism 1
Rhamnose containing glycans 1
linker unit-arabinogalactan synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-α-D-galactose biosynthesis 1 1 1
UDP-N-acetyl-D-galactosamine biosynthesis I 1 1 1
UDP-α-D-galactofuranose biosynthesis 2 1 1
diethylphosphate degradation 2 1 1
D-galactose detoxification 3 1 1
D-galactose degradation I (Leloir pathway) 5 2 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 4 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 4 1
stachyose degradation 7 2 1
UDP-sugars interconversion 9 3 1
teichuronic acid biosynthesis (B. subtilis 168) 9 2 1
colanic acid building blocks biosynthesis 11 11 1
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 18 1 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 12 1