Experiment set4IT027 for Rhodospirillum rubrum S1H
L-Malic Acid carbon source with ammonium chloride; no light
Group: anaerboic nitrogen sourceMedia: MELiSSA_defined_noCarbon_noNitrogen_phosphatebuffered + Ammonium chloride (35.33 mM) + L-Malic acid (20 mM), pH=7
Culturing: Rubrum_ML4a, serum bottle, Anaerobic, at 30 (C)
By: Kelly Wetmore; Jordan Baker on 4/22/19
Media components: 0.051 mM Manganese (II) chloride tetrahydrate, 0.0811 mM Magnesium Sulfate Heptahydrate, 3.801 mM Sodium sulfate, 0.34 mM Calcium chloride dihydrate, 0.068 mM EDTA, 3.601 mM Potassium phosphate monobasic, 0.072 mM Iron (II) sulfate heptahydrate, 50 mM Sodium bicarbonate, 2.985 mM Potassium phosphate dibasic, 1.902 uM Nickel (II) sulfate hexahydrate, 0.348 uM Zinc sulfate heptahydrate, 0.02 uM Copper (II) sulfate pentahydrate, 1.623 uM Boric Acid, 0.207 uM Sodium Molybdate Dihydrate, 0.06 uM biotin, 10 mM Potassium phosphate monobasic, 0.597 mM Potassium phosphate dibasic
Specific Phenotypes
For 4 genes in this experiment
For anaerboic nitrogen source Ammonium chloride in Rhodospirillum rubrum S1H
For anaerboic nitrogen source Ammonium chloride across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Galactose metabolism
- gamma-Hexachlorocyclohexane degradation
- Nucleotide sugars metabolism
- Folate biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: