LB with 5-Hydroxymethylfurfural 2.5 mM
Group:
stress
Media:
LB +
5-Hydroxymethylfurfural (2.5 mM) +
Dimethyl Sulfoxide (0.107142857 vol%)
Culturing: korea_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Jordan on
10/22/2014
Media components: 10 g/L
Tryptone, 5 g/L
Yeast Extract, 5 g/L
Sodium Chloride
Growth plate: 1020 C5,C6
Specific Phenotypes
For 1 genes in this experiment
For stress 5-Hydroxymethylfurfural in Sphingomonas koreensis DSMZ 15582
For stress 5-Hydroxymethylfurfural across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|
| benzoyl-CoA biosynthesis | 3 | 3 | 1 |
| acrylate degradation II | 3 | 1 | 1 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 9 | 3 |
| adipate degradation | 5 | 4 | 1 |
| adipate biosynthesis | 5 | 4 | 1 |
| glutaryl-CoA degradation | 5 | 3 | 1 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 3 | 1 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 3 | 1 |
| acrylate degradation I | 5 | 3 | 1 |
| propanoyl-CoA degradation II | 5 | 3 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 1 | 1 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 2 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 2 |
| oleate β-oxidation | 35 | 30 | 6 |
| fatty acid salvage | 6 | 5 | 1 |
| L-isoleucine degradation I | 6 | 4 | 1 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 1 |
| β-alanine biosynthesis II | 6 | 4 | 1 |
| methyl ketone biosynthesis (engineered) | 6 | 3 | 1 |
| propanoate fermentation to 2-methylbutanoate | 6 | 3 | 1 |
| fatty acid β-oxidation I (generic) | 7 | 5 | 1 |
| pyruvate fermentation to butanoate | 7 | 3 | 1 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 3 | 1 |
| benzoyl-CoA degradation I (aerobic) | 7 | 2 | 1 |
| L-valine degradation I | 8 | 5 | 1 |
| pyruvate fermentation to butanol I | 8 | 3 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 12 | 2 |
| valproate β-oxidation | 9 | 6 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 1 |
| phenylacetate degradation I (aerobic) | 9 | 3 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 8 | 1 |
| 3-phenylpropanoate degradation | 10 | 6 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 4 | 1 |
| superpathway of phenylethylamine degradation | 11 | 4 | 1 |
| gallate degradation III (anaerobic) | 11 | 3 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 4 | 2 |
| L-glutamate degradation VII (to butanoate) | 12 | 4 | 1 |
| 3-hydroxypropanoate cycle | 13 | 9 | 1 |
| glyoxylate assimilation | 13 | 6 | 1 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 4 | 1 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 2 | 1 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 12 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 2 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 6 | 1 |
| glycerol degradation to butanol | 16 | 10 | 1 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 4 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 6 | 1 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 4 | 1 |
| superpathway of the 3-hydroxypropanoate cycle | 18 | 10 | 1 |
| toluene degradation VI (anaerobic) | 18 | 4 | 1 |
| platensimycin biosynthesis | 26 | 6 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |