Experiment set4IT027 for Dinoroseobacter shibae DFL-12
marine broth with Cisplatin 0.05 mg/ml
Group: stressMedia: marine_broth_2216 + Cisplatin (0.05 mg/ml)
Culturing: Dino_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 2/27/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 826 C3,C4
Specific Phenotypes
For 10 genes in this experiment
For stress Cisplatin in Dinoroseobacter shibae DFL-12
For stress Cisplatin across organisms
SEED Subsystems
Subsystem | #Specific |
---|---|
DNA-replication | 2 |
DNA repair, UvrABC system | 2 |
DNA repair, bacterial RecFOR pathway | 2 |
DNA Repair Base Excision | 1 |
DNA repair, bacterial | 1 |
Transcription factors bacterial | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes: