Experiment set4IT025 for Pseudomonas fluorescens FW300-N2C3

LB with Rifampicin 0.0024 mg/ml

Group: stress
Media: LB + Rifampicin (0.0024 mg/ml)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/6/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 982 A3,A4

Specific Phenotypes

For 14 genes in this experiment

For stress Rifampicin in Pseudomonas fluorescens FW300-N2C3

For stress Rifampicin across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
Oxidative stress	1
Peptidoglycan Biosynthesis	1
Siderophore Pyoverdine	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Puromycin biosynthesis
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
queuosine biosynthesis I (de novo)	4	4	1
queuosine biosynthesis III (queuosine salvage)	5	3	1