Experiment set4IT025 for Dinoroseobacter shibae DFL-12
marine broth with Paraquat dichloride 0.015 mg/ml
Group: stressMedia: marine_broth_2216 + Paraquat dichloride (0.015 mg/ml)
Culturing: Dino_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 2/27/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 826 B7,B8
Specific Phenotypes
For 4 genes in this experiment
For stress Paraquat dichloride in Dinoroseobacter shibae DFL-12
For stress Paraquat dichloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glycerol and Glycerol-3-phosphate Uptake and Utilization | 4 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycerophosphodiester degradation | 2 | 2 | 1 |
| phosphatidylcholine resynthesis via glycerophosphocholine | 2 | 1 | 1 |
| glycerol and glycerophosphodiester degradation | 4 | 3 | 1 |
| phospholipid remodeling (phosphatidylethanolamine, yeast) | 4 | 2 | 1 |
| anandamide biosynthesis I | 12 | 3 | 1 |