Experiment set4IT022 for Pseudomonas fluorescens FW300-N2C3

LB with Doxycycline hyclate 0.0001 mg/ml

Group: stress
Media: LB + Doxycycline hyclate (1e-04 mg/ml)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/6/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 985 E5,E6

Specific Phenotypes

For 23 genes in this experiment

For stress Doxycycline hyclate in Pseudomonas fluorescens FW300-N2C3

For stress Doxycycline hyclate across organisms

SEED Subsystems

Subsystem	#Specific
Oxidative stress	3
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
Arginine and Ornithine Degradation	1
Glutathione: Redox cycle	1
Lipid A modifications	1
Methionine Biosynthesis	1
Methionine Degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Benzoxazinone biosynthesis
• Lipopolysaccharide biosynthesis
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Fructose and mannose metabolism
• Ubiquinone and menaquinone biosynthesis
• Glycine, serine and threonine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Glutathione metabolism
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Keratan sulfate biosynthesis
• Peptidoglycan biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Diterpenoid biosynthesis
• Zeatin biosynthesis
• Phenylpropanoid biosynthesis
• Flavone and flavonol biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
poly-β-1,6-N-acetyl-D-glucosamine biosynthesis	4	3	2
glutathione-peroxide redox reactions	3	2	1