Experiment set4IT022 for Azospirillum brasilense Sp245

inner cut, LB soft agar motility assay

Group: motility
Media: LB + Agar (3 g/L)
Culturing: AzoBra_ML2a, Agar plate, Aerobic, at 30 (C), shaken=0 rpm, (Solid)
By: Mark on 5/20/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 5 genes in this experiment

For motility Agar in Azospirillum brasilense Sp245

For motility Agar across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial hemoglobins	1
Carotenoids	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Biosynthesis of terpenoids and steroids
• Biosynthesis of plant hormones
• Biosynthesis of steroids
• Carotenoid biosynthesis - General
• Biosynthesis of alkaloids derived from terpenoid and polyketide

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
epoxysqualene biosynthesis	3	2	2
neurosporene biosynthesis	5	2	2
trans-lycopene biosynthesis I	6	2	2
trans-lycopene biosynthesis II (oxygenic phototrophs and green sulfur bacteria)	8	3	2
β-carotene biosynthesis (engineered)	8	2	2
lycopadiene biosynthesis	7	1	1
cholesterol biosynthesis III (via desmosterol)	16	2	2
cholesterol biosynthesis II (via 24,25-dihydrolanosterol)	16	2	2
cholesterol biosynthesis I	16	2	2
botryococcenes and methylated squalene biosynthesis	9	1	1
staphyloxanthin biosynthesis	9	1	1
4,4'-diapolycopenedioate biosynthesis	9	1	1
superpathway of carotenoid biosynthesis in plants	22	3	2
superpathway of ergosterol biosynthesis II	26	10	2
superpathway of ergosterol biosynthesis I	26	5	2
hopanoid biosynthesis (bacteria)	14	5	1
superpathway of cholesterol biosynthesis	38	5	2