Experiment set4IT019 for Rhodanobacter denitrificans FW104-10B01

R2A with Calcium chloride dihydrate 250 mM

Group: stress
Media: R2A + Calcium chloride dihydrate (250 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans Carlson and Trenton Owens on 06/22/2021
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 3 E4

Specific Phenotypes

For 19 genes in this experiment

For stress Calcium chloride dihydrate in Rhodanobacter denitrificans FW104-10B01

For stress Calcium chloride dihydrate across organisms

SEED Subsystems

Subsystem	#Specific
ATP-dependent RNA helicases, bacterial	1
DNA-replication	1
Heat shock dnaK gene cluster extended	1
Hfl operon	1
Nudix proteins (nucleoside triphosphate hydrolases)	1
Polyadenylation bacterial	1
Protein chaperones	1
Terminal cytochrome C oxidases	1
Ton and Tol transport systems	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Oxidative phosphorylation
• Purine metabolism
• Puromycin biosynthesis
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Folate biosynthesis
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arsenite to oxygen electron transfer	2	1	1
arsenite to oxygen electron transfer (via azurin)	3	1	1
aerobic respiration I (cytochrome c)	4	3	1
aerobic respiration II (cytochrome c) (yeast)	4	2	1
Fe(II) oxidation	6	2	1