Experiment set4IT019 for Dinoroseobacter shibae DFL-12

Compare to:

marine broth with Benzethonium chloride 0.008 mM

Group: stress
Media: marine_broth_2216 + Benzethonium chloride (0.008 mM)
Culturing: Dino_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 2/27/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 827 E7,E8

Specific Phenotypes

For 22 genes in this experiment

For stress Benzethonium chloride in Dinoroseobacter shibae DFL-12

For stress Benzethonium chloride across organisms

SEED Subsystems

Subsystem #Specific
Widespread colonization island 2
Biogenesis of c-type cytochromes 1
Isobutyryl-CoA to Propionyl-CoA Module 1
Periplasmic disulfide interchange 1
Terminal cytochrome C oxidases 1
Valine degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
10,13-epoxy-11-methyl-octadecadienoate biosynthesis 2 2 2
cis-cyclopropane fatty acid (CFA) biosynthesis 1 1 1
sterculate biosynthesis 2 1 1
arsenite to oxygen electron transfer 2 1 1
benzoyl-CoA biosynthesis 3 3 1
arsenite to oxygen electron transfer (via azurin) 3 1 1
aerobic respiration I (cytochrome c) 4 3 1
aerobic respiration II (cytochrome c) (yeast) 4 2 1
2-methyl-branched fatty acid β-oxidation 14 10 3
adipate degradation 5 5 1
fatty acid β-oxidation IV (unsaturated, even number) 5 4 1
adipate biosynthesis 5 4 1
fatty acid β-oxidation II (plant peroxisome) 5 3 1
propanoyl-CoA degradation II 5 3 1
acrylate degradation I 5 2 1
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) 5 2 1
(8E,10E)-dodeca-8,10-dienol biosynthesis 11 5 2
β-alanine biosynthesis II 6 4 1
methyl ketone biosynthesis (engineered) 6 3 1
Fe(II) oxidation 6 2 1
fatty acid β-oxidation I (generic) 7 5 1
fatty acid β-oxidation VI (mammalian peroxisome) 7 4 1
benzoyl-CoA degradation I (aerobic) 7 3 1
L-valine degradation I 8 5 1
phenylacetate degradation I (aerobic) 9 6 1
valproate β-oxidation 9 5 1
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 4 1
superpathway of coenzyme A biosynthesis II (plants) 10 8 1
3-phenylpropanoate degradation 10 4 1
superpathway of phenylethylamine degradation 11 6 1
Spodoptera littoralis pheromone biosynthesis 22 3 2
oleate β-oxidation 35 28 3
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 2 1
superpathway of glyoxylate cycle and fatty acid degradation 14 12 1
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 2 1
platensimycin biosynthesis 26 6 1
mycolate biosynthesis 205 21 3
superpathway of mycolate biosynthesis 239 22 3