Experiment set4IT017 for Pseudomonas fluorescens FW300-N2C3

LB with Bacitracin 2 mg/ml

Group: stress
Media: LB + Bacitracin (2 mg/ml)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/6/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 984 E3,E4

Specific Phenotypes

For 7 genes in this experiment

For stress Bacitracin in Pseudomonas fluorescens FW300-N2C3

For stress Bacitracin across organisms

SEED Subsystems

Subsystem	#Specific
CBSS-562.2.peg.5158 SK3 including	1
Cobalt-zinc-cadmium resistance	1
DNA repair, bacterial	1
Lipid A modifications	1
Peptidoglycan Biosynthesis	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Glycine, serine and threonine metabolism
• Lysine degradation
• Benzoxazinone biosynthesis
• Biotin metabolism
• Diterpenoid biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1