Experiment set4IT014 for Caulobacter crescentus NA1000
PYE with Polymyxin B sulfate 0.005 mg/ml
Group: stressMedia: PYE + Polymyxin B sulfate (0.005 mg/ml)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.0 generations
By: Adam on 1-May-17
Media components: 2 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.8 mM Magnesium sulfate, 0.5 mM Calcium chloride
Growth plate: 1709 D2
Specific Phenotypes
For 21 genes in this experiment
For stress Polymyxin B sulfate in Caulobacter crescentus NA1000
For stress Polymyxin B sulfate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Flagellum | 1 |
| Flagellum in Campylobacter | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| thiosulfate disproportionation IV (rhodanese) | 1 | 1 | 1 |
| L-cysteine degradation III | 2 | 1 | 1 |
| tRNA-uridine 2-thiolation (mammalian mitochondria) | 4 | 2 | 1 |
| tRNA-uridine 2-thiolation (yeast mitochondria) | 4 | 2 | 1 |
| sulfide oxidation IV (mitochondria) | 5 | 2 | 1 |
| tRNA-uridine 2-thiolation (cytoplasmic) | 8 | 2 | 1 |