Experiment set4IT014 for Bacteroides thetaiotaomicron VPI-5482

BHIS with Fusidic acid sodium salt 0.004 mg/ml

Group: stress
Media: BHIS + Fusidic acid sodium salt (0.004 mg/ml)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 11-Oct-17
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin
Growth plate: Btheta_sp4, copyB C2

Specific Phenotypes

For 17 genes in this experiment

For stress Fusidic acid sodium salt in Bacteroides thetaiotaomicron VPI-5482

For stress Fusidic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Histidine Degradation	2
KDO2-Lipid A biosynthesis	1
Potassium homeostasis	1
Protein chaperones	1
Purine Utilization	1
RNA processing and degradation, bacterial	1
Two cell division clusters relating to chromosome partitioning	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Histidine metabolism
• Purine metabolism
• Glutamate metabolism
• Lipopolysaccharide biosynthesis
• Nitrogen metabolism
• Drug metabolism - other enzymes
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine degradation I	1	1	1
L-glutamate biosynthesis I	2	2	1
L-histidine degradation I	4	3	2
L-histidine degradation II	5	3	2
L-histidine degradation III	6	6	2
ammonia assimilation cycle III	3	3	1
guanosine ribonucleotides de novo biosynthesis	4	4	1
L-histidine degradation VI	8	7	2
L-asparagine biosynthesis III (tRNA-dependent)	4	1	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	1	1
lipid IVA biosynthesis (P. gingivalis)	9	9	2
superpathway of guanosine nucleotides de novo biosynthesis I	6	6	1
lipid IVA biosynthesis (H. pylori)	6	6	1
lipid IVA biosynthesis (Vibrio cholerae serogroup O1 El Tor)	6	5	1
lipid IVA biosynthesis (E. coli)	6	5	1
lipid IVA biosynthesis (generic)	6	5	1
lipid IVA biosynthesis (P. putida)	6	5	1
L-glutamate and L-glutamine biosynthesis	7	5	1
superpathway of guanosine nucleotides de novo biosynthesis II	8	7	1
L-citrulline biosynthesis	8	5	1
superpathway of L-citrulline metabolism	12	8	1
superpathway of purine nucleotide salvage	14	13	1
superpathway of (Kdo)2-lipid A biosynthesis	17	11	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	1
superpathway of Kdo2-lipid A biosynthesis	25	11	1
superpathway of purine nucleotides de novo biosynthesis II	26	23	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	42	1