Experiment set4IT012 for Pseudomonas fluorescens FW300-N2C3

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LB with Benzalkonium Chloride 0.016 mg/ml

Group: stress
Media: LB + Benzalkonium Chloride (0.016 mg/ml)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/6/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 984 B3,B4

Specific Phenotypes

For 52 genes in this experiment

For stress Benzalkonium Chloride in Pseudomonas fluorescens FW300-N2C3

For stress Benzalkonium Chloride across organisms

SEED Subsystems

Subsystem #Specific
Oxidative stress 4
ABC transporter alkylphosphonate (TC 3.A.1.9.1) 3
ABC transporter oligopeptide (TC 3.A.1.5.1) 2
Arginine and Ornithine Degradation 2
DNA repair, UvrABC system 2
Lipid A modifications 2
Polyamine Metabolism 2
Terminal cytochrome O ubiquinol oxidase 2
Terminal cytochrome oxidases 2
Acid resistance mechanisms 1
Bacterial Cytoskeleton 1
Choline and Betaine Uptake and Betaine Biosynthesis 1
DNA-binding regulatory proteins, strays 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Methionine Biosynthesis 1
Methionine Degradation 1
Orphan regulatory proteins 1
Periplasmic disulfide interchange 1
Photorespiration (oxidative C2 cycle) 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Serine Biosynthesis 1
Staphylococcal pathogenicity islands SaPI 1
Threonine and Homoserine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
arginine dependent acid resistance 1 1 1
3-(4-hydroxyphenyl)pyruvate biosynthesis 1 1 1
L-aspartate biosynthesis 1 1 1
L-aspartate degradation I 1 1 1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway) 2 2 1
NADH to cytochrome bo oxidase electron transfer I 2 2 1
succinate to cytochrome bo oxidase electron transfer 2 2 1
superoxide radicals degradation 2 2 1
NADH to cytochrome bo oxidase electron transfer II 2 2 1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer 2 2 1
proline to cytochrome bo oxidase electron transfer 2 2 1
putrescine biosynthesis I 2 2 1
L-glutamate degradation II 2 2 1
L-tyrosine degradation II 2 1 1
L-tryptophan degradation IV (via indole-3-lactate) 2 1 1
atromentin biosynthesis 2 1 1
oleate biosynthesis II (animals and fungi) 2 1 1
pyruvate to cytochrome bo oxidase electron transfer 2 1 1
malate/L-aspartate shuttle pathway 2 1 1
methanol oxidation to formaldehyde IV 2 1 1
D-lactate to cytochrome bo oxidase electron transfer 2 1 1
palmitoleate biosynthesis IV (fungi and animals) 2 1 1
L-phenylalanine biosynthesis I 3 3 1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) 3 3 1
L-tyrosine biosynthesis I 3 3 1
ethanol degradation IV 3 3 1
L-serine biosynthesis I 3 3 1
putrescine biosynthesis II 3 3 1
L-phenylalanine degradation II (anaerobic) 3 2 1
L-cysteine biosynthesis IX (Trichomonas vaginalis) 3 2 1
L-asparagine degradation III (mammalian) 3 2 1
(R)-cysteate degradation 3 1 1
sulfolactate degradation III 3 1 1
indole-3-acetate biosynthesis VI (bacteria) 3 1 1
L-tyrosine degradation IV (to 4-methylphenol) 3 1 1
reactive oxygen species degradation 4 4 1
superpathway of L-serine and glycine biosynthesis I 4 4 1
superpathway of putrescine biosynthesis 4 3 1
superpathway of L-aspartate and L-asparagine biosynthesis 4 3 1
L-tyrosine degradation III 4 2 1
L-phenylalanine degradation III 4 2 1
L-tryptophan degradation VIII (to tryptophol) 4 1 1
spermidine biosynthesis III 4 1 1
adipate degradation 5 5 1
L-tyrosine degradation I 5 5 1
trans-4-hydroxy-L-proline degradation I 5 3 1
superpathway of plastoquinol biosynthesis 5 2 1
L-tyrosine degradation V (reductive Stickland reaction) 5 1 1
4-hydroxybenzoate biosynthesis I (eukaryotes) 5 1 1
L-phenylalanine degradation VI (reductive Stickland reaction) 5 1 1
L-tryptophan degradation XIII (reductive Stickland reaction) 5 1 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 6 2
fatty acid salvage 6 6 1
superpathway of L-threonine biosynthesis 6 6 1
TCA cycle VIII (Chlamydia) 6 4 1
superpathway of sulfolactate degradation 6 2 1
coenzyme M biosynthesis II 6 1 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 2
anaerobic energy metabolism (invertebrates, cytosol) 7 4 1
superpathway of polyamine biosynthesis II 8 5 1
superpathway of polyamine biosynthesis I 8 5 1
sorgoleone biosynthesis 8 2 1
superpathway of aromatic amino acid biosynthesis 18 18 2
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 4 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
rosmarinic acid biosynthesis I 10 3 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 10 1
(S)-reticuline biosynthesis I 11 1 1
oleate β-oxidation 35 30 3
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 12 1
indole-3-acetate biosynthesis II 12 5 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of L-arginine and L-ornithine degradation 13 12 1
2-methyl-branched fatty acid β-oxidation 14 11 1
superpathway of rosmarinic acid biosynthesis 14 4 1
superpathway of arginine and polyamine biosynthesis 17 14 1
superpathway of anaerobic energy metabolism (invertebrates) 17 8 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
aspartate superpathway 25 22 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 3 1
superpathway of chorismate metabolism 59 44 2
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1