Experiment set4IT010 for Rhodanobacter denitrificans FW104-10B01
R2A with Magnesium sulfate 3.90625 mM
Group: stressMedia: R2A + Magnesium sulfate (3.90625 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 28 (C), shaken=700 rpm
By: Hans Carlson and Trenton Owens on 22-Jun-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 2 D9
Specific Phenotypes
For 2 genes in this experiment
For stress Magnesium sulfate in Rhodanobacter denitrificans FW104-10B01
For stress Magnesium sulfate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Methionine Salvage | 1 |
| Queuosine-Archaeosine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| 5'-deoxyadenosine degradation I | 3 | 2 | 1 |
| 5'-deoxyadenosine degradation II | 4 | 1 | 1 |
| S-methyl-5-thio-α-D-ribose 1-phosphate degradation II | 5 | 1 | 1 |
| S-methyl-5-thio-α-D-ribose 1-phosphate degradation III | 5 | 1 | 1 |
| S-methyl-5-thio-α-D-ribose 1-phosphate degradation I | 7 | 6 | 1 |
| L-methionine salvage cycle III | 11 | 10 | 1 |
| L-methionine salvage cycle II (plants) | 11 | 7 | 1 |
| L-methionine salvage cycle I (bacteria and plants) | 12 | 9 | 1 |