Experiment set4IT010 for Methanococcus maripaludis JJ
Formate N-free with 2mM Adenosine
Group: nitrogen sourceMedia: Mc_formate_N_Free + Adenosine (2 mM), pH=7
Culturing: Methanococcus_JJ_ML1, 96 deep-well microplate; 1 mL volume, Anaerobic, at 37 (C)
By: Leslie Day on 2-Dec-22
Media components: 2.5 g/L Sodium bicarbonate, 10.5 g/L Sodium Chloride, 0.14 g/L Potassium phosphate dibasic, 0.0095 g/L Iron (II) chloride tetrahydrate, 0.001 g/L resazurin sodium salt, 0.84 g/L Sodium acetate, 13.6 g/L Sodium Formate, 0.5 g/L Dithiothreitol, 0.001 g/L Sodium sulfide nonahydrate, MOPS_2M_pH7 (0.002 M 3-(N-morpholino)propanesulfonic acid, 0.0005 M Sodium Hydroxide), Mc_general_salts_SO4free (0.335 g/L Potassium Chloride, 5.6 g/L Magnesium chloride hexahydrate, 0.105 g/L Calcium chloride), Mc_trace_minerals_SO4free (0.021 g/L Trisodium citrate dihydrate, 0.005 g/L Manganese (II) chloride tetrahydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc chloride, 0.0001 g/L copper (II) chloride dihydrate, 0.0001 g/L Aluminum chloride hydrate, 0.0001 g/L Boric Acid, 0.001 g/L Sodium Molybdate Dihydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.002 g/L Sodium selenate, 0.0001 g/L vanadium chloride, 3.3e-05 g/L Sodium tungstate dihydrate), Mc_vitamins (2e-05 g/L biotin, 2e-05 g/L Folic Acid, 0.0001 g/L Pyridoxine HCl, 5e-05 g/L Thiamine HCl, 5e-05 g/L Riboflavin, 5e-05 g/L Nicotinic Acid, 5e-05 g/L calcium pantothenate, 1e-06 g/L Cyanocobalamin, 5e-05 g/L 4-Aminobenzoic acid, 5e-05 g/L Lipoic acid)
Specific Phenotypes
For 22 genes in this experiment
For nitrogen source Adenosine in Methanococcus maripaludis JJ
For nitrogen source Adenosine across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Folate biosynthesis
- Purine metabolism
- Histidine metabolism
- Tyrosine metabolism
- Tryptophan metabolism
- Lipopolysaccharide biosynthesis
- Carotenoid biosynthesis - General
- Anthocyanin biosynthesis
- Alkaloid biosynthesis I
- Fructose and mannose metabolism
- Fatty acid biosynthesis
- Ubiquinone and menaquinone biosynthesis
- Lysine degradation
- Phenylalanine metabolism
- Benzoxazinone biosynthesis
- Glycerophospholipid metabolism
- Ether lipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- 1- and 2-Methylnaphthalene degradation
- Tetrachloroethene degradation
- Naphthalene and anthracene degradation
- Benzoate degradation via CoA ligation
- Ethylbenzene degradation
- Butanoate metabolism
- Porphyrin and chlorophyll metabolism
- Limonene and pinene degradation
- Diterpenoid biosynthesis
- Nitrogen metabolism
- Phenylpropanoid biosynthesis
- Flavonoid biosynthesis
- Alkaloid biosynthesis II
- Insect hormone biosynthesis
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of siderophore group nonribosomal peptides
- Biosynthesis of type II polyketide backbone
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: