Experiment set4IT009 for Pseudomonas fluorescens SBW25-INTG

N-Acetyl-D-Glucosamine nitrogen source; with MOPS

Group: nitrogen source
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + N-Acetyl-D-Glucosamine (10 mM) + 3-(N-morpholino)propanesulfonic acid (40 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 4 genes in this experiment

For nitrogen source D-Glucose in Pseudomonas fluorescens SBW25-INTG

For nitrogen source D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	3
Chitin and N-acetylglucosamine utilization	1
Sialic Acid Metabolism	1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
alkylnitronates degradation	2	2	1
N-acetylglucosamine degradation I	2	2	1
nitrate reduction V (assimilatory)	2	1	1
N-acetylglucosamine degradation II	3	2	1
D-galactosamine and N-acetyl-D-galactosamine degradation	4	2	1
N-acetyl-D-galactosamine degradation	5	2	1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation	6	2	1
chitin derivatives degradation	8	2	1
peptidoglycan recycling I	14	11	1
superpathway of N-acetylneuraminate degradation	22	14	1