Experiment set4IT007 for Pseudomonas fluorescens SBW25-INTG

N-Acetyl-D-Glucosamine nitrogen source; with MOPS

Group: nitrogen source
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + N-Acetyl-D-Glucosamine (10 mM) + 3-(N-morpholino)propanesulfonic acid (40 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 6 genes in this experiment

For nitrogen source D-Glucose in Pseudomonas fluorescens SBW25-INTG

For nitrogen source D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	3

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
alkylnitronates degradation	2	2	1
nitrate reduction V (assimilatory)	2	1	1
glycolysis IV	10	8	1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle	22	18	1