Experiment set4IT005 for Pseudomonas fluorescens FW300-N2C3

LB with Sodium Chlorite 0.08 mM

Group: stress
Media: LB + Sodium Chlorite (0.08 mM)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/6/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 983 D3,D4

Specific Phenotypes

For 38 genes in this experiment

For stress Sodium Chlorite in Pseudomonas fluorescens FW300-N2C3

For stress Sodium Chlorite across organisms

SEED Subsystems

Subsystem	#Specific
Siderophore Pyoverdine	8
Transport of Iron	6
Iron acquisition in Vibrio	5
Molybdenum cofactor biosynthesis	3
Terminal cytochrome oxidases	3
Campylobacter Iron Metabolism	2
Terminal cytochrome d ubiquinol oxidases	2
Terminal cytochrome O ubiquinol oxidase	1
Transport of Molybdenum	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Caffeine metabolism
• Methionine metabolism
• Lysine biosynthesis
• Tyrosine metabolism
• Tryptophan metabolism
• Glutathione metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Sphingolipid metabolism
• 1,4-Dichlorobenzene degradation
• Riboflavin metabolism
• Nicotinate and nicotinamide metabolism
• Porphyrin and chlorophyll metabolism
• Terpenoid biosynthesis
• Carotenoid biosynthesis - General
• Nitrogen metabolism
• Caprolactam degradation
• Biosynthesis of terpenoids and steroids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
succinate to cytochrome bo oxidase electron transfer	2	2	1
proline to cytochrome bo oxidase electron transfer	2	2	1
NADH to cytochrome bo oxidase electron transfer I	2	2	1
NADH to cytochrome bo oxidase electron transfer II	2	2	1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer	2	2	1
ferrichrome biosynthesis	2	1	1
D-lactate to cytochrome bo oxidase electron transfer	2	1	1
pyruvate to cytochrome bo oxidase electron transfer	2	1	1
aerobic respiration III (alternative oxidase pathway)	3	3	1
heme a biosynthesis	4	2	1
ferrichrome A biosynthesis	5	2	1
pyoverdine I biosynthesis	11	7	1
mycobactin biosynthesis	11	3	1