Experiment set4IT004 for Paraburkholderia graminis OAS925
1-aminocyclopropane-1-carboxylate (ACC) nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_noNitrogen + 1-aminocyclopropane-1-carboxylate (3 mM)
Culturing: Burkholderia_OAS925_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Marta on 20-May-21
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 7 genes in this experiment
For nitrogen source 1-aminocyclopropane-1-carboxylate in Paraburkholderia graminis OAS925
For nitrogen source 1-aminocyclopropane-1-carboxylate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Lactate utilization | 3 |
| Glycolate, glyoxylate interconversions | 2 |
| Photorespiration (oxidative C2 cycle) | 2 |
| L-rhamnose utilization | 1 |
| Peptidoglycan Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycolate and glyoxylate degradation II | 2 | 2 | 1 |
| methylglyoxal degradation V | 3 | 2 | 1 |
| glycolate and glyoxylate degradation III | 3 | 1 | 1 |
| glycolate and glyoxylate degradation I | 4 | 3 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 4 | 2 |
| superpathway of glycol metabolism and degradation | 7 | 5 | 1 |
| lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) | 8 | 4 | 1 |
| photorespiration I | 9 | 6 | 1 |
| photorespiration III | 9 | 6 | 1 |
| photorespiration II | 10 | 6 | 1 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 1 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 1 |