Experiment set4IT004 for Bacteroides thetaiotaomicron VPI-5482
BHIS with Vancomycin Hydrochloride Hydrate 0.016 mg/ml
Group: stressMedia: BHIS + Vancomycin Hydrochloride Hydrate (0.016 mg/ml)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 11-Oct-17
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin
Growth plate: Btheta_sp5, copyA A2
Specific Phenotypes
For 5 genes in this experiment
For stress Vancomycin Hydrochloride Hydrate in Bacteroides thetaiotaomicron VPI-5482
For stress Vancomycin Hydrochloride Hydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Peptidoglycan Biosynthesis | 1 |
| Peptidyl-prolyl cis-trans isomerase | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycolysis / Gluconeogenesis
- Peptidoglycan biosynthesis
- Pyruvate metabolism
- Propanoate metabolism
- Reductive carboxylate cycle (CO2 fixation)
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| acetate and ATP formation from acetyl-CoA III | 1 | 1 | 1 |
| acetate conversion to acetyl-CoA | 1 | 1 | 1 |
| superpathway of acetate utilization and formation | 3 | 3 | 1 |
| ethanol degradation II | 3 | 2 | 1 |
| ethanol degradation IV | 3 | 2 | 1 |
| L-isoleucine biosynthesis V | 3 | 2 | 1 |
| ethanol degradation III | 3 | 1 | 1 |
| chitin deacetylation | 4 | 1 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 4 | 2 |
| superpathway of bitter acids biosynthesis | 18 | 3 | 3 |
| colupulone and cohumulone biosynthesis | 6 | 1 | 1 |
| adlupulone and adhumulone biosynthesis | 6 | 1 | 1 |
| lupulone and humulone biosynthesis | 6 | 1 | 1 |
| reductive glycine pathway of autotrophic CO2 fixation | 9 | 6 | 1 |
| cis-geranyl-CoA degradation | 9 | 1 | 1 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 1 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 1 |