Experiment set4IT003 for Rhodanobacter denitrificans FW104-10B01
R2A with ammonium iron(II) sulfate hexahydrate 2.5 mM
Group: stressMedia: R2A + ammonium iron(II) sulfate hexahydrate (2.5 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 28 (C), shaken=700 rpm
By: Hans Carlson and Trenton Owens on 06/22/2021
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1 D3
Specific Phenotypes
For 6 genes in this experiment
For stress ammonium iron(II) sulfate hexahydrate in Rhodanobacter denitrificans FW104-10B01
For stress ammonium iron(II) sulfate hexahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Peptidoglycan Biosynthesis | 1 |
| Type IV pilus | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 11 | 2 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 11 | 2 |
| peptidoglycan biosynthesis V (β-lactam resistance) | 17 | 10 | 2 |
| peptidoglycan biosynthesis I (meso-diaminopimelate containing) | 12 | 10 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 4 | 1 |
| peptidoglycan biosynthesis III (mycobacteria) | 15 | 10 | 1 |