Experiment set4IT001 for Pseudomonas fluorescens FW300-N2C3

LB with Sodium nitrite 10 mM

Group: stress
Media: LB + Sodium nitrite (10 mM)
Culturing: pseudo5_N2-C3_1_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on 10/6/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 983 A1,A2

Specific Phenotypes

For 7 genes in this experiment

For stress Sodium nitrite in Pseudomonas fluorescens FW300-N2C3

For stress Sodium nitrite across organisms

SEED Subsystems

Subsystem	#Specific
Terminal cytochrome O ubiquinol oxidase	4
Terminal cytochrome oxidases	4
Biogenesis of cbb3-type cytochrome c oxidases	1
Carboxysome	1
Cyanate hydrolysis	1
Terminal cytochrome C oxidases	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Methionine metabolism
• Tryptophan metabolism
• Riboflavin metabolism
• Porphyrin and chlorophyll metabolism
• Terpenoid biosynthesis
• Carotenoid biosynthesis - General
• Nitrogen metabolism
• Biosynthesis of terpenoids and steroids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
CO2 fixation into oxaloacetate (anaplerotic)	2	2	1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer	2	2	1
succinate to cytochrome bo oxidase electron transfer	2	2	1
NADH to cytochrome bo oxidase electron transfer II	2	2	1
proline to cytochrome bo oxidase electron transfer	2	2	1
NADH to cytochrome bo oxidase electron transfer I	2	2	1
D-lactate to cytochrome bo oxidase electron transfer	2	1	1
pyruvate to cytochrome bo oxidase electron transfer	2	1	1
cyanate degradation	3	3	1
heme a biosynthesis	4	2	1
C4 photosynthetic carbon assimilation cycle, NADP-ME type	7	4	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	6	1
3-hydroxypropanoate cycle	13	5	1
glyoxylate assimilation	13	4	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	1
gluconeogenesis II (Methanobacterium thermoautotrophicum)	18	9	1
3-hydroxypropanoate/4-hydroxybutanate cycle	18	8	1
superpathway of the 3-hydroxypropanoate cycle	18	5	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	21	1