Experiment set4IT001 for Bacteroides thetaiotaomicron VPI-5482

BHIS with Dimethyl Sulfoxide 9 vol%

Group: stress
Media: BHIS + Dimethyl Sulfoxide (9 vol%)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 11-Oct-17
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin
Growth plate: Btheta_sp4, copyA D3

Specific Phenotypes

For 7 genes in this experiment

For stress Dimethyl Sulfoxide in Bacteroides thetaiotaomicron VPI-5482

For stress Dimethyl Sulfoxide across organisms

SEED Subsystems

Subsystem	#Specific
Copper homeostasis: copper tolerance	1
Phosphate metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lysine degradation
• Peptidoglycan biosynthesis
• Biotin metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan recycling II	10	3	1
peptidoglycan recycling I	14	8	1