Experiment set4H48 for Pseudomonas stutzeri RCH2

LB with Polymyxin B sulfate 0.0001 mg/ml

Group: stress
Media: LB + Polymyxin B sulfate (1e-04 mg/ml)
Culturing: psRCH2_ML7, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 6/28/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 562 B3,B4

Specific Phenotypes

For 11 genes in this experiment

For stress Polymyxin B sulfate in Pseudomonas stutzeri RCH2

For stress Polymyxin B sulfate across organisms

SEED Subsystems

Subsystem	#Specific
Cobalt-zinc-cadmium resistance	1
De Novo Purine Biosynthesis	1
Pentose phosphate pathway	1
Ribosome biogenesis bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
PRPP biosynthesis	1	1	1
(1,4)-β-D-xylan degradation	2	1	1
cellulose and hemicellulose degradation (cellulolosome)	3	2	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	44	1