Experiment set4H46 for Pseudomonas stutzeri RCH2

LB with Gentamicin sulfate salt 0.0016 mg/ml

Group: stress
Media: LB + Gentamicin sulfate salt (0.0016 mg/ml)
Culturing: psRCH2_ML7, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 6/28/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 562 A1,A2

Specific Phenotypes

For 31 genes in this experiment

For stress Gentamicin sulfate salt in Pseudomonas stutzeri RCH2

For stress Gentamicin sulfate salt across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial Chemotaxis	3
Flagellar motility	2
Cobalt-zinc-cadmium resistance	1
Flagellum	1
Glutathione-regulated potassium-efflux system and associated functions	1
Glycine cleavage system	1
Iron acquisition in Vibrio	1
N-linked Glycosylation in Bacteria	1
Nudix proteins (nucleoside triphosphate hydrolases)	1
Orphan regulatory proteins	1
Potassium homeostasis	1
Protein chaperones	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Tyrosine metabolism
• Benzoate degradation via hydroxylation
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Nucleotide sugars metabolism
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
adenosine nucleotides degradation III	1	1	1
Arg/N-end rule pathway (eukaryotic)	14	8	4
vitamin K-epoxide cycle	4	1	1
UDP-N-acetyl-β-L-fucosamine biosynthesis	4	1	1
UDP-N-acetyl-β-L-quinovosamine biosynthesis	4	1	1
CMP-pseudaminate biosynthesis	6	6	1
superpathway of photosynthetic hydrogen production	6	2	1
CMP-diacetamido-8-epilegionaminic acid biosynthesis	7	1	1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis	24	9	1