Experiment set4H30 for Shewanella amazonensis SB2B

LB with Bacitracin 0.25 mg/ml

Group: stress
Media: LB + Bacitracin (0.25 mg/ml)
Culturing: SB2B_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Adam on 7/26/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 590 E1,E2

Specific Phenotypes

For 28 genes in this experiment

For stress Bacitracin in Shewanella amazonensis SB2B

For stress Bacitracin across organisms

SEED Subsystems

Subsystem	#Specific
Transport of Zinc	3
Multidrug Resistance Efflux Pumps	2
Bacterial Cytoskeleton	1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	1
Peptidoglycan Biosynthesis	1
Ribonucleotide reduction	1
Ribosome biogenesis bacterial	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lipopolysaccharide biosynthesis
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Methionine metabolism
• Tryptophan metabolism
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Folate biosynthesis
• Biosynthesis of siderophore group nonribosomal peptides

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I	7	2	2
L-methionine salvage cycle III	11	6	2
L-methionine salvage cycle II (plants)	11	4	2
L-methionine salvage cycle I (bacteria and plants)	12	6	2