Experiment set4H3 for Pseudomonas stutzeri RCH2

LB with Fusidic acid sodium salt 0.016 mg/ml

Group: stress
Media: LB + Fusidic acid sodium salt (0.016 mg/ml)
Culturing: psRCH2_ML7, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 6/24/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 557 E5,E6

Specific Phenotypes

For 30 genes in this experiment

For stress Fusidic acid sodium salt in Pseudomonas stutzeri RCH2

For stress Fusidic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Cobalt-zinc-cadmium resistance	2
DNA repair, UvrABC system	2
Proteolysis in bacteria, ATP-dependent	2
Bacterial Chemotaxis	1
Central meta-cleavage pathway of aromatic compound degradation	1
Control of cell elongation - division cycle in Bacilli	1
DNA Repair Base Excision	1
Hemin transport system	1
Malonate decarboxylase	1
Peptidoglycan Biosynthesis	1
Proteasome bacterial	1
Purine conversions	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Fructose and mannose metabolism
• Fatty acid biosynthesis
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Nucleotide sugars metabolism
• Keratan sulfate biosynthesis
• Lipopolysaccharide biosynthesis
• Peptidoglycan biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
fatty acid biosynthesis initiation (type II)	3	3	1
malonate degradation I (biotin-independent)	3	3	1
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis	3	1	1
purine deoxyribonucleosides degradation II	3	1	1
adenine and adenosine salvage V	3	1	1
fatty acid biosynthesis initiation (mitochondria)	4	2	1
adenine and adenosine salvage III	4	2	1
purine deoxyribonucleosides degradation I	4	1	1
fatty acid biosynthesis initiation (plant mitochondria)	4	1	1
superpathway of fatty acid biosynthesis initiation	5	4	1
adenosine nucleotides degradation II	5	4	1
purine ribonucleosides degradation	6	1	1
superpathway of purine deoxyribonucleosides degradation	7	2	1
pederin biosynthesis	14	2	2
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis	8	1	1
bryostatin biosynthesis	19	2	2
purine nucleotides degradation II (aerobic)	11	8	1
mycobactin biosynthesis	11	1	1
superpathway of fatty acid biosynthesis I (E. coli)	16	15	1
mupirocin biosynthesis	26	2	1
corallopyronin A biosynthesis	30	2	1
superpathway of fatty acid biosynthesis II (plant)	43	38	1
superpathway of fatty acids biosynthesis (E. coli)	53	51	1