Culturing: SB2B_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
| Pathway | #Steps | #Present | #Specific |
|---|
| benzoyl-CoA biosynthesis | 3 | 3 | 3 |
| L-glutamate biosynthesis I | 2 | 2 | 2 |
| fatty acid β-oxidation III (unsaturated, odd number) | 1 | 1 | 1 |
| L-glutamine degradation I | 1 | 1 | 1 |
| L-glutamine degradation II | 1 | 1 | 1 |
| fatty acid β-oxidation I (generic) | 7 | 6 | 6 |
| oleate β-oxidation | 35 | 33 | 27 |
| ammonia assimilation cycle III | 3 | 3 | 2 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 4 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 7 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 3 | 3 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 3 | 3 |
| glutaryl-CoA degradation | 5 | 3 | 3 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 9 | 8 |
| valproate β-oxidation | 9 | 6 | 5 |
| oleate β-oxidation (thioesterase-dependent, yeast) | 2 | 2 | 1 |
| fatty acid salvage | 6 | 5 | 3 |
| L-isoleucine degradation I | 6 | 4 | 3 |
| propanoate fermentation to 2-methylbutanoate | 6 | 3 | 3 |
| acetoacetate degradation (to acetyl CoA) | 2 | 1 | 1 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 5 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 3 | 3 |
| pyruvate fermentation to butanoate | 7 | 3 | 3 |
| (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) | 5 | 5 | 2 |
| adipate degradation | 5 | 4 | 2 |
| 5,6-dehydrokavain biosynthesis (engineered) | 10 | 6 | 4 |
| adipate biosynthesis | 5 | 3 | 2 |
| 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) | 10 | 4 | 4 |
| 4-hydroxybenzoate biosynthesis III (plants) | 5 | 2 | 2 |
| fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) | 5 | 2 | 2 |
| pyruvate fermentation to butanol I | 8 | 3 | 3 |
| 5-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP | 3 | 3 | 1 |
| adeninyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP | 3 | 3 | 1 |
| 5-methoxybenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP | 3 | 3 | 1 |
| ketolysis | 3 | 3 | 1 |
| 5-methoxy-6-methylbenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP | 3 | 3 | 1 |
| 5-hydroxybenzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP | 3 | 3 | 1 |
| benzimidazolyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP | 3 | 3 | 1 |
| adenosylcobalamin biosynthesis from adenosylcobinamide-GDP I | 3 | 3 | 1 |
| 2-methyladeninyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP | 3 | 3 | 1 |
| polyhydroxybutanoate biosynthesis | 3 | 2 | 1 |
| 4-methylphenyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP | 3 | 2 | 1 |
| phenyl adenosylcobamide biosynthesis from adenosylcobinamide-GDP | 3 | 2 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 3 |
| methyl ketone biosynthesis (engineered) | 6 | 3 | 2 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 3 |
| oleate β-oxidation (reductase-dependent, yeast) | 3 | 1 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 5 | 3 |
| 3-phenylpropanoate degradation | 10 | 4 | 3 |
| L-glutamate and L-glutamine biosynthesis | 7 | 6 | 2 |
| benzoyl-CoA degradation I (aerobic) | 7 | 2 | 2 |
| L-valine degradation I | 8 | 6 | 2 |
| (2S)-ethylmalonyl-CoA biosynthesis | 4 | 2 | 1 |
| adenosylcobalamin biosynthesis from adenosylcobinamide-GDP II | 4 | 2 | 1 |
| L-glutamate degradation VII (to butanoate) | 12 | 3 | 3 |
| 2-methylpropene degradation | 8 | 2 | 2 |
| glutaminyl-tRNAgln biosynthesis via transamidation | 4 | 1 | 1 |
| oleate β-oxidation (isomerase-dependent, yeast) | 4 | 1 | 1 |
| L-asparagine biosynthesis III (tRNA-dependent) | 4 | 1 | 1 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 5 | 3 |
| phenylacetate degradation I (aerobic) | 9 | 2 | 2 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 11 | 3 |
| ketogenesis | 5 | 3 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 4 | 3 |
| androstenedione degradation I (aerobic) | 25 | 6 | 5 |
| methyl tert-butyl ether degradation | 10 | 2 | 2 |
| fatty acid β-oxidation VII (yeast peroxisome) | 5 | 1 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 1 | 1 |
| pyruvate fermentation to acetone | 5 | 1 | 1 |
| ethylbenzene degradation (anaerobic) | 5 | 1 | 1 |
| isopropanol biosynthesis (engineered) | 5 | 1 | 1 |
| glycerol degradation to butanol | 16 | 9 | 3 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 3 | 3 |
| superpathway of phenylethylamine degradation | 11 | 3 | 2 |
| superpathway of testosterone and androsterone degradation | 28 | 6 | 5 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 7 | 3 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 3 | 3 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 6 | 3 |
| 6-gingerol analog biosynthesis (engineered) | 6 | 2 | 1 |
| superpathway of cholesterol degradation I (cholesterol oxidase) | 42 | 8 | 7 |
| toluene degradation VI (anaerobic) | 18 | 3 | 3 |
| 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) | 12 | 2 | 2 |
| 10-cis-heptadecenoyl-CoA degradation (yeast) | 12 | 2 | 2 |
| 4-ethylphenol degradation (anaerobic) | 6 | 1 | 1 |
| 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) | 6 | 1 | 1 |
| jasmonic acid biosynthesis | 19 | 4 | 3 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 2 | 2 |
| superpathway of cholesterol degradation II (cholesterol dehydrogenase) | 47 | 8 | 7 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 20 | 4 |
| androstenedione degradation II (anaerobic) | 27 | 4 | 4 |
| acetyl-CoA fermentation to butanoate | 7 | 4 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 2 | 2 |
| mevalonate pathway I (eukaryotes and bacteria) | 7 | 1 | 1 |
| mevalonate pathway II (haloarchaea) | 7 | 1 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 4 | 3 |
| superpathway of adenosylcobalamin salvage from cobinamide I | 8 | 8 | 1 |
| L-citrulline biosynthesis | 8 | 7 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 3 | 1 |
| isoprene biosynthesis II (engineered) | 8 | 1 | 1 |
| mevalonate pathway IV (archaea) | 8 | 1 | 1 |
| mevalonate pathway III (Thermoplasma) | 8 | 1 | 1 |
| cholesterol degradation to androstenedione I (cholesterol oxidase) | 17 | 2 | 2 |
| platensimycin biosynthesis | 26 | 6 | 3 |
| superpathway of adenosylcobalamin salvage from cobinamide II | 9 | 8 | 1 |
| 4-oxopentanoate degradation | 9 | 2 | 1 |
| NiFe(CO)(CN)2 cofactor biosynthesis | 10 | 5 | 1 |
| superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) | 10 | 4 | 1 |
| L-lysine fermentation to acetate and butanoate | 10 | 3 | 1 |
| gallate degradation III (anaerobic) | 11 | 3 | 1 |
| ethylmalonyl-CoA pathway | 11 | 2 | 1 |
| cholesterol degradation to androstenedione II (cholesterol dehydrogenase) | 22 | 2 | 2 |
| superpathway of L-citrulline metabolism | 12 | 9 | 1 |
| superpathway of cholesterol degradation III (oxidase) | 49 | 4 | 4 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 21 | 2 |
| sitosterol degradation to androstenedione | 18 | 1 | 1 |
| superpathway of ergosterol biosynthesis I | 26 | 3 | 1 |
| adenosylcobalamin biosynthesis II (aerobic) | 33 | 18 | 1 |
| adenosylcobalamin biosynthesis I (anaerobic) | 36 | 18 | 1 |
| superpathway of cholesterol biosynthesis | 38 | 3 | 1 |
| superpathway of L-lysine degradation | 43 | 8 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 22 | 1 |