Experiment set4H28 for Marinobacter adhaerens HP15
marine broth with 1-ethyl-3-methylimidazolium chloride 7.5 mM
Group: stressMedia: marine_broth_2216 + 1-ethyl-3-methylimidazolium chloride (7.5 mM)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 10/23/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 761 E3,E4
Specific Phenotypes
For 17 genes in this experiment
For stress 1-ethyl-3-methylimidazolium chloride in Marinobacter adhaerens HP15
For stress 1-ethyl-3-methylimidazolium chloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Polyamine Metabolism | 5 |
| Ribosome biogenesis bacterial | 2 |
| ZZ gjo need homes | 2 |
| Ubiquinone Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Tryptophan metabolism
- Biosynthesis of alkaloids derived from shikimate pathway
- Ubiquinone and menaquinone biosynthesis
- Urea cycle and metabolism of amino groups
- Pyrimidine metabolism
- Methionine metabolism
- Arginine and proline metabolism
- Histidine metabolism
- Phenylalanine metabolism
- gamma-Hexachlorocyclohexane degradation
- Bisphenol A degradation
- Cyanoamino acid metabolism
- Toluene and xylene degradation
- 1- and 2-Methylnaphthalene degradation
- Naphthalene and anthracene degradation
- 1,4-Dichlorobenzene degradation
- Benzoate degradation via CoA ligation
- Styrene degradation
- Methane metabolism
- Indole and ipecac alkaloid biosynthesis
- Limonene and pinene degradation
- Brassinosteroid biosynthesis
- Carotenoid biosynthesis - General
- Phenylpropanoid biosynthesis
- Flavonoid biosynthesis
- Isoflavonoid biosynthesis
- Alkaloid biosynthesis I
- Biosynthesis of siderophore group nonribosomal peptides
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| spermidine biosynthesis I | 2 | 2 | 1 |
| spermine biosynthesis | 2 | 1 | 1 |
| pseudouridine degradation | 2 | 1 | 1 |
| aminopropylcadaverine biosynthesis | 3 | 2 | 1 |
| spermidine biosynthesis III | 4 | 2 | 1 |
| superpathway of polyamine biosynthesis II | 8 | 6 | 1 |
| superpathway of polyamine biosynthesis I | 8 | 5 | 1 |
| L-methionine salvage cycle III | 11 | 9 | 1 |
| L-methionine salvage cycle I (bacteria and plants) | 12 | 8 | 1 |
| superpathway of arginine and polyamine biosynthesis | 17 | 14 | 1 |
| secologanin and strictosidine biosynthesis | 17 | 1 | 1 |