Experiment set4H26 for Marinobacter adhaerens HP15

marine broth with 1-ethyl-3-methylimidazolium acetate 15 mM

Group: stress
Media: marine_broth_2216 + 1-ethyl-3-methylimidazolium acetate (15 mM)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 10/23/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 761 D3,D4

Specific Phenotypes

For 11 genes in this experiment

For stress 1-ethyl-3-methylimidazolium acetate in Marinobacter adhaerens HP15

For stress 1-ethyl-3-methylimidazolium acetate across organisms

SEED Subsystems

Subsystem	#Specific
De Novo Pyrimidine Synthesis	1
Glutathione: Non-redox reactions	1
Homogentisate pathway of aromatic compound degradation	1
Methylglyoxal Metabolism	1
Pyrimidine utilization	1
Two cell division clusters relating to chromosome partitioning	1
Ubiquinone Biosynthesis	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Histidine metabolism
• Phenylalanine metabolism
• gamma-Hexachlorocyclohexane degradation
• Bisphenol A degradation
• Tryptophan metabolism
• Cyanoamino acid metabolism
• Pyruvate metabolism
• Toluene and xylene degradation
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• 1,4-Dichlorobenzene degradation
• Benzoate degradation via CoA ligation
• Styrene degradation
• Methane metabolism
• Limonene and pinene degradation
• Brassinosteroid biosynthesis
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Isoflavonoid biosynthesis
• Alkaloid biosynthesis I
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
methylglyoxal degradation VIII	3	2	1
methylglyoxal degradation I	3	2	1
superpathway of methylglyoxal degradation	8	4	1