Experiment set4H18 for Phaeobacter inhibens DSM 17395
marine broth
Group: marine brothMedia: marine_broth_2216
Culturing: Phaeo_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jordan on 8/6/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 623 C1,D1
Specific Phenotypes
For 12 genes in this experiment
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fructose and mannose metabolism
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Peptidoglycan biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-sorbitol degradation I | 3 | 3 | 1 |
| sucrose degradation I (sucrose phosphotransferase) | 3 | 1 | 1 |
| sucrose degradation IV (sucrose phosphorylase) | 4 | 3 | 1 |
| sucrose degradation III (sucrose invertase) | 4 | 3 | 1 |
| sucrose degradation VII (sucrose 3-dehydrogenase) | 4 | 1 | 1 |
| sucrose degradation II (sucrose synthase) | 5 | 4 | 1 |
| mannitol cycle | 5 | 3 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 3 | 2 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 1 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 1 |
| heterolactic fermentation | 18 | 13 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 14 | 1 |