Experiment set4H17 for Marinobacter adhaerens HP15

marine broth with 1-ethyl-3-methylimidazolium lysine 5 mM

Group: stress
Media: marine_broth_2216 + 1-ethyl-3-methylimidazolium lysine (5 mM)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 10/23/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 760 F5,F6

Specific Phenotypes

For 1 genes in this experiment

For stress 1-ethyl-3-methylimidazolium lysine in Marinobacter adhaerens HP15

For stress 1-ethyl-3-methylimidazolium lysine across organisms

SEED Subsystems

Subsystem	#Specific
Arginine and Ornithine Degradation	1
Polyamine Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	3	1
putrescine biosynthesis II	3	3	1
superpathway of polyamine biosynthesis II	8	6	1