Experiment set4H14 for Marinobacter adhaerens HP15
marine broth with Cholin acetate 76.57 mM
Group: stressMedia: marine_broth_2216 + Cholin acetate (76.57 mM)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 10/23/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 760 E1,E2
Specific Phenotypes
For 7 genes in this experiment
For stress Cholin acetate in Marinobacter adhaerens HP15
For stress Cholin acetate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Choline and Betaine Uptake and Betaine Biosynthesis | 2 |
| DNA-binding regulatory proteins, strays | 1 |
| Oxidative stress | 1 |
| Purine Utilization | 1 |
| Thioredoxin-disulfide reductase | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycine betaine biosynthesis I (Gram-negative bacteria) | 2 | 2 | 2 |
| choline degradation I | 2 | 2 | 2 |
| glycine betaine biosynthesis II (Gram-positive bacteria) | 2 | 2 | 2 |
| choline-O-sulfate degradation | 3 | 2 | 2 |
| β-alanine biosynthesis I | 2 | 1 | 1 |
| β-alanine biosynthesis IV | 2 | 1 | 1 |
| glycine betaine biosynthesis III (plants) | 3 | 2 | 1 |
| dimethylsulfoniopropanoate biosynthesis I (Wollastonia) | 3 | 1 | 1 |
| choline degradation IV | 4 | 2 | 1 |
| dimethylsulfoniopropanoate biosynthesis II (Spartina) | 4 | 1 | 1 |