Experiment set4H13 for Shewanella amazonensis SB2B

LB with Sodium nitrate 200 mM

Group: stress
Media: LB + Sodium nitrate (200 mM)
Culturing: SB2B_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Adam on 7/26/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 589 A7,A8

Specific Phenotypes

For 18 genes in this experiment

For stress Sodium nitrate in Shewanella amazonensis SB2B

For stress Sodium nitrate across organisms

SEED Subsystems

Subsystem	#Specific
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Glutathione metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
icosapentaenoate biosynthesis IV (bacteria)	1	1	1
gondoate biosynthesis (anaerobic)	4	4	1
palmitate biosynthesis II (type II fatty acid synthase)	31	29	7
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)	9	9	2
oleate biosynthesis IV (anaerobic)	14	13	3
superpathway of fatty acids biosynthesis (E. coli)	53	50	11
superpathway of unsaturated fatty acids biosynthesis (E. coli)	20	20	4
cis-vaccenate biosynthesis	5	5	1
8-amino-7-oxononanoate biosynthesis IV	5	4	1
fatty acid elongation -- saturated	5	4	1
superpathway of fatty acid biosynthesis II (plant)	43	37	8
8-amino-7-oxononanoate biosynthesis I	11	10	2
anteiso-branched-chain fatty acid biosynthesis	34	24	6
even iso-branched-chain fatty acid biosynthesis	34	24	6
odd iso-branched-chain fatty acid biosynthesis	34	24	6
(5Z)-dodecenoate biosynthesis I	6	6	1
stearate biosynthesis II (bacteria and plants)	6	5	1
(5Z)-dodecenoate biosynthesis II	6	5	1
streptorubin B biosynthesis	34	20	5
biotin biosynthesis I	15	14	2
superpathway of fatty acid biosynthesis I (E. coli)	16	13	2
mycolate biosynthesis	205	20	4
superpathway of mycolate biosynthesis	239	21	4