Experiment set3S939 for Rhodanobacter sp. FW510-T8

Compare to:

NLDM_defined_nocarbon with D-Glucose

Group: no nutrient control
Media: NLDM_defined_nocarbon + D-Glucose (20 mM), pH=7
Culturing: rhodanobacter_T8_ML1, 96 well deep well block, Aerobic, at 23 (C), shaken=700 rpm
By: Hira Lesea on 09/01/2025
Media components: 1 mM Ammonium chloride, 3.3 mM Potassium Chloride, 0.812 mM Magnesium Sulfate Heptahydrate, 0.68 mM Calcium chloride dihydrate, 4.05 mM Disodium phosphate, 0.95 mM Sodium phosphate monobasic, ATCC Wolfe's mineral mix (5 mg/L EDTA, 3 mg/L Magnesium Sulfate Heptahydrate, 5 mg/L Manganese (II) sulfate monohydrate, 10 mg/L Sodium Chloride, 1 mg/L Iron (II) sulfate heptahydrate, 1 mg/L Cobalt(II) nitrate hexahydrate, 1 mg/L Calcium chloride dihydrate, 1 mg/L Zinc sulfate heptahydrate, 0.1 mg/L Copper (II) sulfate pentahydrate, 0.1 mg/L Aluminum potassium sulfate dodecahydrate, 0.1 mg/L Boric Acid, 0.1 mg/L Sodium Molybdate Dihydrate, 0.01 mg/L Sodium selenite pentahydrate, 0.1 mg/L Sodium tungstate dihydrate, 0.2 mg/L Nickel (II) chloride hexahydrate), ATCC Wolfe's vitamin mix (20 ug/L Folic Acid, 100 ug/L Pyridoxine HCl, 50 ug/L Riboflavin, 20 ug/L biotin, 50 ug/L Thiamine HCl, 50 ug/L Nicotinic Acid, 50 ug/L calcium pantothenate, 1 ug/L Cyanocobalamin, 50 ug/L 4-Aminobenzoic acid, 50 ug/L Lipoic acid)

Specific Phenotypes

For 7 genes in this experiment

For no nutrient control D-Glucose in Rhodanobacter sp. FW510-T8

For no nutrient control D-Glucose across organisms

SEED Subsystems

Subsystem #Specific
Cysteine Biosynthesis 3
Methionine Biosynthesis 3
DNA-binding regulatory proteins, strays 1
Flagellum 1
Flagellum in Campylobacter 1
One-carbon metabolism by tetrahydropterines 1
Serine-glyoxylate cycle 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
assimilatory sulfate reduction III 3 3 2
assimilatory sulfate reduction I 4 4 2
L-homocysteine biosynthesis 2 1 1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) 10 8 4
superpathway of L-cysteine biosynthesis (fungi) 6 4 2
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 10 3
assimilatory sulfate reduction IV 4 3 1
homocysteine and cysteine interconversion 4 3 1
L-methionine biosynthesis III 4 2 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 8 2
L-methionine biosynthesis I 5 2 1
L-methionine biosynthesis II 6 4 1
folate transformations I 13 8 2
superpathway of L-homoserine and L-methionine biosynthesis 8 5 1
folate transformations III (E. coli) 9 9 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 6 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 6 1
reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) 10 3 1
folate transformations II (plants) 11 10 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 12 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 15 1
aspartate superpathway 25 21 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 20 1