Experiment set3S931 for Rhodanobacter sp. FW510-T8

Compare to:

NLDM_defined_nocarbon with D-Glucose and 20AA_mix 0.1X

Group: nutrient
Media: NLDM_defined_nocarbon + D-Glucose (20 mM) + 0.1X 20AA_mix, pH=7
Culturing: rhodanobacter_T8_ML1, 96 well deep well block, Aerobic, at 23 (C), shaken=700 rpm
By: Hira Lesea on 09/01/2025
Media components: 1 mM Ammonium chloride, 3.3 mM Potassium Chloride, 0.812 mM Magnesium Sulfate Heptahydrate, 0.68 mM Calcium chloride dihydrate, 4.05 mM Disodium phosphate, 0.95 mM Sodium phosphate monobasic, ATCC Wolfe's mineral mix (5 mg/L EDTA, 3 mg/L Magnesium Sulfate Heptahydrate, 5 mg/L Manganese (II) sulfate monohydrate, 10 mg/L Sodium Chloride, 1 mg/L Iron (II) sulfate heptahydrate, 1 mg/L Cobalt(II) nitrate hexahydrate, 1 mg/L Calcium chloride dihydrate, 1 mg/L Zinc sulfate heptahydrate, 0.1 mg/L Copper (II) sulfate pentahydrate, 0.1 mg/L Aluminum potassium sulfate dodecahydrate, 0.1 mg/L Boric Acid, 0.1 mg/L Sodium Molybdate Dihydrate, 0.01 mg/L Sodium selenite pentahydrate, 0.1 mg/L Sodium tungstate dihydrate, 0.2 mg/L Nickel (II) chloride hexahydrate), ATCC Wolfe's vitamin mix (20 ug/L Folic Acid, 100 ug/L Pyridoxine HCl, 50 ug/L Riboflavin, 20 ug/L biotin, 50 ug/L Thiamine HCl, 50 ug/L Nicotinic Acid, 50 ug/L calcium pantothenate, 1 ug/L Cyanocobalamin, 50 ug/L 4-Aminobenzoic acid, 50 ug/L Lipoic acid)

20AA_mix 0.1x includes: 0.05 mM L-Arginine, 0.05 mM L-Histidine, 0.05 mM L-Lysine, 0.05 mM L-Aspartic Acid, 0.05 mM L-Glutamic acid monopotassium salt monohydrate, 0.05 mM L-Serine, 0.05 mM L-Threonine, 0.05 mM L-Asparagine, 0.05 mM L-Glutamine, 0.05 mM L-Cysteine hydrochloride monohydrate, 0.05 mM Glycine, 0.05 mM L-Proline, 0.05 mM L-Alanine, 0.05 mM L-Valine, 0.05 mM L-Isoleucine, 0.05 mM L-Leucine, 0.05 mM L-Methionine, 0.05 mM L-Phenylalanine, 0.05 mM L-tyrosine disodium salt, 0.05 mM L-Tryptophan (final concentrations)

Specific Phenotypes

For 3 genes in this experiment

For nutrient D-Glucose in Rhodanobacter sp. FW510-T8

For nutrient D-Glucose across organisms

SEED Subsystems

Subsystem #Specific
Multidrug Resistance Efflux Pumps 1
Serine-glyoxylate cycle 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glyoxylate cycle 6 6 1
superpathway of glyoxylate bypass and TCA 12 10 1
superpathway of glyoxylate cycle and fatty acid degradation 14 11 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 23 1