Experiment set3S709 for Pseudomonas aeruginosa PA14

Compare to:

Condition=Reactor_with_PCN_75uM; Oxidizing_potential_applied=yes; Time=5_days; Collection=outgrowth_in_LB

Group: survival
Media: DM + Condition=Reactor_with_PCN_75uM; Oxidizing_potential_applied=yes; Time=5_days; Collection=outgrowth_in_LB
Culturing: Paeruginosa_PA14_ML18, at 33 (C), shaken=150 rpm
By: Kemal on 6/25/25
Media components: 100 mM 3-(N-morpholino)propanesulfonic acid, 43 mM Sodium Chloride, 93 mM Ammonium chloride, 3.7 mM Potassium phosphate monobasic, 1 mM Magnesium sulfate, 0.02 mM D-Glucose

Specific Phenotypes

For 12 genes in this experiment

For survival Condition=Reactor_with_PCN_75uM; Oxidizing_potential_applied=yes; Time=5_days; Collection=outgrowth_in_LB in Pseudomonas aeruginosa PA14

For survival Condition=Reactor_with_PCN_75uM; Oxidizing_potential_applied=yes; Time=5_days; Collection=outgrowth_in_LB across organisms

SEED Subsystems

Subsystem #Specific
Proteolysis in bacteria, ATP-dependent 2
Cysteine Biosynthesis 1
Ethanolamine utilization 1
Experimental tye 1
Fermentations: Lactate 1
Fermentations: Mixed acid 1
Fructose utilization 1
Heme and Siroheme Biosynthesis 1
MLST 1
Propanediol utilization 1
Proteasome bacterial 1
Protein chaperones 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
Threonine anaerobic catabolism gene cluster 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate and ATP formation from acetyl-CoA I 2 2 1
sulfoacetaldehyde degradation I 2 1 1
pyruvate fermentation to acetate II 3 3 1
superpathway of acetate utilization and formation 3 3 1
pyruvate fermentation to acetate IV 3 2 1
pyruvate fermentation to acetate VII 3 2 1
pyruvate fermentation to acetate I 3 2 1
heme b biosynthesis II (oxygen-independent) 4 3 1
pyruvate fermentation to acetate and (S)-lactate I 4 3 1
pyruvate fermentation to acetate and lactate II 4 2 1
sulfolactate degradation II 4 1 1
ethanolamine utilization 5 5 1
acetylene degradation (anaerobic) 5 4 1
(S)-propane-1,2-diol degradation 5 3 1
L-threonine degradation I 6 5 1
superpathway of heme b biosynthesis from uroporphyrinogen-III 6 4 1
superpathway of taurine degradation 6 2 1
methanogenesis from acetate 6 2 1
superpathway of sulfolactate degradation 6 2 1
acetyl-CoA fermentation to butanoate 7 3 1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) 8 4 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 5 1
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 5 1
superpathway of L-alanine fermentation (Stickland reaction) 9 4 1
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) 9 3 1
L-lysine fermentation to acetate and butanoate 10 3 1
gallate degradation III (anaerobic) 11 3 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 6 1
mixed acid fermentation 16 13 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 7 1
heterolactic fermentation 18 14 1
superpathway of L-threonine metabolism 18 14 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 13 1
superpathway of methanogenesis 21 2 1
superpathway of N-acetylneuraminate degradation 22 15 1
purine nucleobases degradation II (anaerobic) 24 16 1
superpathway of bacteriochlorophyll a biosynthesis 26 6 1
superpathway of L-lysine degradation 43 15 1