Experiment set3S522 for Rhodopseudomonas palustris CGA009
Sodium acetate trihydrate and Sodium L-Lactate carbon source
Group: carbon sourceMedia: PM + Sodium acetate trihydrate (10 mM) + Sodium L-Lactate (13 mM) + Light intensity (12 µmol photons/m2/s from a 60-W incandescent light bulb)
Culturing: RPal_CGA009_ML8, tube, Anaerobic, at 30 (C)
By: Rpal_McKinlay on 9/8/24
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 1 g/L Ammonium Sulfate, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, UW concentrated base (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium sulfate, 0.00667 g/L Calcium chloride dihydrate, 1.85e-05 g/L ammonium molybdate tetrahydrate, 0.000698 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc sulfate heptahydrate, 0.000154 g/L Manganese (II) sulfate monohydrate, 3.92e-05 g/L Copper (II) sulfate pentahydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)
Specific Phenotypes
For 3 genes in this experiment
For carbon source Sodium acetate trihydrate in Rhodopseudomonas palustris CGA009
For carbon source Sodium acetate trihydrate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| methylglyoxal degradation VIII | 3 | 3 | 1 |
| methylglyoxal degradation I | 3 | 2 | 1 |
| methylglyoxal degradation V | 3 | 1 | 1 |
| lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) | 8 | 3 | 1 |
| superpathway of methylglyoxal degradation | 8 | 3 | 1 |